寨卡检测试剂盒

英文名称：Zika Virus    (Real time) DOUBLE CHECK

品牌：Genekam

规格：100T

Principle and use

This amplification kit has been manufactured by Genekam Biotechnology AG, Germany to detect Zika-Virus (Double check. This is an absolute quantification assay. It tests twice the samples.

Real time PCR is based on fluorogenic dyes. In our kit we use 2 dyes (2 probes), first Probe they are 6-Carboxy tetramethyl rhodamine (quencher) and Carboxy-fluorescein (reporter; called also FAM-Channel). Second Probe is labelled with HEX reporter (Filter for is VIC or in some machines, it is HEX)on one side and on the other side 6-Carboxy tetramethyl rhodamine (quencher). Up to 40 Ct for each dye should be taken positive. Value between 40-45 Ct for each dye should be taken as marginal positive (doubtful).

Composition:

It contains the following: (Warning: THAW THE TUBES SLOWLY: NEVER THAW IN HEATING BLOCK OR WITH HEAT FROM HAND):

? Tube A (2 tubes)

? Tube B (2 tubes)
? Tube Y (1 tube)

? Positive (+Ve) control (D1): to be stored at -20°C (1 tube): It must be used with pipette tips with filter or it should be used with separate pipettor.

? Negative (-Ve) Control (tube D2) (1 tube)

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